Comparative Immunological Analysis of Host Plasma Proteins Bound to Bloodstream Forms of Trypanosoma brucei Subspecies
نویسندگان
چکیده
منابع مشابه
Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei.
With the completion of the sequence of the megabase hromosomes of Trypanosoma brucei [1], it has become traightforward to design primers and amplify the appropriate anking sequences to knock out genes by homologous recomination [2]. In addition, RNA interference can be used to own-regulate individual genes or gene families [3], and has lso been applied to genome-wide screens [4]. Despite these ...
متن کاملThe selenoproteome is dispensable in bloodstream forms of Trypanosoma brucei.
Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei. Both life cycle stages of T. brucei are highly sensitive to auranofin, a compound known to target selenoproteins. However, the same sensitivity is observed in the SepSecS double knock...
متن کاملIdentification of subspecies specific genes differentially expressed in procyclic forms of Trypanosoma brucei subspecies.
Trypanosoma brucei subspecies undergo establishment and maturation in tsetse flies mid-gut and salivary glands, respectively. Successful establishment of trypanosomes in tsetse mid-gut as well as their migration to saliva gland depends on the ability of these parasites to adapt rapidly to new environmental conditions and to negotiate the physical barriers. To identify subspecies specific genes ...
متن کاملIn vitro growth inhibition of bloodstream forms of Trypanosoma brucei and Trypanosoma congolense by iron chelators
African trypanosomes exert significant morbidity and mortality in man and livestock. Only a few drugs are available for the treatment of trypanosome infections and therefore, the development of new anti-trypanosomal agents is required. Previously it has been shown that bloodstream-form trypanosomes are sensitive to the iron chelator deferoxamine. In this study the effect of 13 iron chelators on...
متن کاملEthiopian strains of Trypanosoma brucei subspecies.
SINCE 1967 we have isolated 6 strains of Trypanosoma (Trypanozoon) brucei subspecies from various hosts in Illubabor Province, Ethiopia, where human trypanosomiasis was first recognised in 1967 and where it became epidemic in 1968-1970 (McConnell Hutchinson & Baker, 1970; Hutchinson, 1971). Some but not all of these isolations have been reported previously; all of those from mammals were made f...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1978
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.21.2.605-612.1978